Abundance Study of Rhizosphere Fungi Based on Polymerase Chain Reaction (PCR) and Its Influence on the Growth and Production of 4 Varieties of Soybean (Glycine Max (L.) Merrill) in Marginal Land of Lampung

EasyChair Preprint 10852

Abstract

In addition to land conditions, microorganism activities such as rhizosphere fungi found in marginal lands can also influence the growth and production of soybean plants (Suharta, 2010). This research aims to determine the differences in viability, vigor, development, and production of 4 soybean varieties cultivated in marginal lands, identify variations in the abundance of rhizosphere fungi among the 4 soybean varieties present in marginal lands, and ascertain the correlation between observed variables of soybean plants and the abundance of rhizosphere fungi. Soil samples were taken from around the roots of the 4 different soybean varieties, namely Argomulyo, Anjasmoro, Devon-1, and Dena-1, each with 4 replications. Each sample was extracted using the Promega (Germany) method. The extracted DNA was amplified using the Polymerase Chain Reaction (PCR) method based on the Internal Transcribed Spacer (ITS) sequence (White et al, 1990). The amplified results were visualized using the capillary electrophoresis digital method. The visualized DNA bands were around 500 bp, consistent with the expected target amplification size variation in the thickness of the visualized bands indicating the abundance of rhizosphere funding. PCR visualization during the germination period showed that the Devon-1 variety had the thickest bands, while the Anjasmoro variety had the thinnest bands. At 100 days after planting, PCR visualization indicated the absence of DNA bands in all four varieties. The variety with the highest viability and vigor index was Devon-1, while the lowest was Anjasmoro. The variety with the highest growth and production was Dena-1, while the lowest was Argomulyo. The correlation between observed variables for each variety and rhizosphere fungi showed a negative correlation.

Keyphrases: DNA, Electrophoresis, Polymerase Chain Reaction (PCR), Rhizosphere Fungi, Soybean

@booklet{EasyChair:10852,
  author    = {Paul B. Timotiwu and Wawan A. Setiawan and Tumiar Katarina B. Manik and Adinda Nurulita Putri and Ketut Perihartini and Dian Anjar Sari},
  title     = {Abundance Study of Rhizosphere Fungi Based on Polymerase Chain Reaction (PCR) and Its Influence on the Growth and Production of 4 Varieties of Soybean (Glycine Max (L.) Merrill) in Marginal Land of Lampung},
  howpublished = {EasyChair Preprint 10852},
  year      = {EasyChair, 2023}}